Protective roles of Pyracantha fortuneana extract on acute renal toxicity induced by cadmium chloride in rats1
Yixin KeI , Kaihang YuII , Weiliang ZengIII , Guojun LianIV
I Graduate student, Department of Health Inspection and Quarantine, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China. Conception and design of the study, acquisition of data, technical procedures, manuscript preparation and writing. II Graduate student, Department of Health Inspection and Quarantine, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China. Technical procedures, acquisition of data. III Graduate student, Department of Health Inspection and Quarantine, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China. Statistical analysis, interpretation of data. IV Associate Professor, Department of Health Inspection and Quarantine, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China. Conception and design of the study, acquisition of data, technical procedures, manuscript preparation and writing, final approval.
Abstract
Purpose: To investigate the protective roles of pyracantha fortune fruit extract (PFE) on acute renal toxicity induced by cadmium chloride (CdCl2) in rats.
Methods: Rats were pretreated with PFE and consecutively injected with CdCl2 (6.5 mg/kg) for 5 days.
Results: The concentration of Cd, kidney weight, malondialdehyde (MDA), and nitric oxide (NO) production were remarkably increased in CdCl2 group as well as the levels of plasma uric acid, urea, and creatinine (P < 0.001). However, the body weight and glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione peroxidase (GR) levels were markedly reduced by CdCl2 treatment (P < 0.001). Histological manifestations of renal tissue showed severely adverse changes. Moreover, CdCl2 treatment significantly decreased the B-cell lymphoma-2 (Bcl-2) expression while increased the Bcl-2-Associated X Protein (Bax), tumor necrosis factor-α (TNF-α) expression (P < 0.001). Additionally, the expression of Nrf2/Keap 1 related proteins Keap-1 gained a significant increase (P < 0.001), whereas the Nrf2, HO-1, γ-GCS, GSH-Px and NQO1 expression decreased by CdCl2 treatment (P < 0.05). These rats were pretreated with PFE to improve the changes caused by CdCl2 treatment.
Conclusion: PFE could protect the kidney against acute renal toxicity induced by CdCl2.
Keywords
Pyracantha. Acute Toxicity. Cadmium Chloride. Apoptosis. Kidney. Rats.
